HiFliQ Ni Advance FPLC Columns
Fastback Ni Advance Resin designed for faster purification of secreted proteins using cell culture supernatant.
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Fastback Ni Advance Resin designed for faster purification of secreted proteins using cell culture supernatant. Nickel ions are carefully loaded onto an agarose matrix via chelating coupled ligand to obtain a stable affinity matrix with a high binding capacity for histidine residues.
Benefits:
- Ideal for secreted proteins and all intracellular protein expression
- Works in your protocol, regardless of sample origin whether eukaryotic (insect, yeast, HEK293 or CHO) and bacteria (E.coli)
- Great results faster with fewer steps than conventional workflow – no need to buffer exchange your conditioned media; simply load clarified culture media directly on to the Ni Advance resin column.
- Keep your buffer in its preferred conditions as Fastback Ni Advance resin is resistant to EDTA (up to 20mM) and DTT (up to 20 mM).
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