Magnetic NHS-activated Resins
Magnetic NHS (N-hydroxysuccinimide)-activated Resin is used to make magnetic adsorbents coupled with molecules containing amine groups.
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Magnetic NHS (N-hydroxysuccinimide)-activated Resin is used to make magnetic adsorbents coupled with molecules containing amine groups. Coupling biospecific ligands to NHS-activated agarose is a successful and well-documented technique. The coupling reaction is spontaneous, rapid and easy to carry out. No toxic chemicals or special equipment is required.
Magnetic NHS activated Resin forms chemically stable amide bonds with ligands containing primary amine groups. The activated NHS group has a long spacer arm (11 atoms) that is particularly helpful in the immobilization of structurally restricted ligands such as proteins and peptides.
This pre-activated resin can be readily employed to make various magnetic affinity chromatography adsorbents for both small scale and large scale purification applications. After binding of target molecules, the magnetic resin can be readily isolated from the biological feedstocks with the aid of a magnet.
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