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ProteinArk

HiFliQ Ni Advance FPLC Columns

Fastback Ni Advance Resin designed for faster purification of secreted proteins using cell culture supernatant.

All prices exclude VAT.
Name & Code
Grouped product items
Code & DescriptionAvailabilityPriceQty
AGX112-Q1NIADV1
1 ml HiFliQ Ni Advance FPLC column (1 x 1 ml)
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POA
POA
AGX112-Q5NIADV1
5 ml HiFliQ Ni Advance FPLC column (1 x 5 ml)
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POA
POA
AGX112-Q1NIADV5
1 ml HiFliQ Ni Advance FPLC columns (5 x 1 ml)
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POA
POA
AGX112-Q5NIADV5
5 ml HiFliQ Ni Advance FPLC columns (5 x 5 ml)
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POA
POA
AGX129-Q10NIADV1
10 ml Ni-Adv HiFLiQ column (16mm x 50 mm)
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POA
POA
AGX129-Q20NIADV1
20 ml Ni-Adv HiFLiQ column (16mm x 100 mm)
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POA
POA
AGX129-Q50NIADV1
50 ml Ni-Adv HiFLiQ column (26mm x 100 mm)
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POA
POA
Product Description

Fastback Ni Advance Resin designed for faster purification of secreted proteins using cell culture supernatant. Nickel ions are carefully loaded onto an agarose matrix via chelating coupled ligand to obtain a stable affinity matrix with a high binding capacity for histidine residues.

Benefits:
  • Ideal for secreted proteins and all intracellular protein expression
  • Works in your protocol, regardless of sample origin whether eukaryotic (insect, yeast, HEK293 or CHO) and bacteria (E.coli)
  • Great results faster with fewer steps than conventional workflow – no need to buffer exchange your conditioned media; simply load clarified culture media directly on to the Ni Advance resin column.
  • Keep your buffer in its preferred conditions as Fastback Ni Advance resin is resistant to EDTA (up to 20mM) and DTT (up to 20 mM).
Technical DataDelivery & Returns
PRICE & AVAILABILITY: Please note that while we are working closely with our suppliers to minimise any global supply chain issues, prices and availability may be subject to change at short notice.