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NEW! TROUBLE-FREE EM STAINING

UA-Zero® Safe to use, safe to handle EM Stain

UA-Zero Trouble Free EM Staining

UA-Zero® EM Stain is a patented solution developed as a substitute for Uranyl Acetate. UA-Zero does not contain any radioactive material and is safe to use, transport and dispose of. It can be used as a direct replacement for Uranyl Acetate with no changes to standard user protocols and delivers high contrast imagery.

  • Uranyl Acetate substitute
  • Non-radioactive, Uranium free
  • No handling, storage or disposal restrictions
  • Image contrast results as good as Uranyl Acetate, if not better
  • No additional processing required
  • Direct replacement for Uranyl Acetate without changing standard user protocols
  • Independently tested and verified

UA-Zero is non-radioactive and Uranium free. Consequently, there are none of the handling, storage or disposal issues associated with Uranyl Acetate.

The ready-to-use lead-free solution can be used as a direct replacement for to Uranyl Acetate without any changes to the standard user protocols, and no additional processing is required to enhance the image contrast. As shown in the images below, the image contrast results carried out at Bristol University are as good as Uranyl Acetate, if not better.

UA-Zero is easy to use; to stain the sample on a TEM grid, with a support film, only a few simple steps are required before drying.

Image Comparisons

Comparison TEM images of HeLa cells grown on coverslips with samples prepared using UA-Zero EM Stain and Uranyl acetate, courtesy of Prof. Dr. Paul Verkade and Dr. Chris Neal, Wolfson Bioimaging Facility, School of Medical Sciences, University of Bristol.

TEM images of sample prepared using UA-Zero EM Stain in 20% ethanol staining solution.

TEM images of sample prepared using UA-Zero EM Stain in 20% ethanol as a staining solution.

TEM images of sample prepared using 3% Uranyl acetate in water staining solution.

TEM images of sample prepared using 3% Uranyl acetate in water as a staining solution.

TEM images of sample prepared using 3% Uranyl acetate in 20% ethanol staining solution.

TEM images of sample prepared using 3% Uranyl acetate in 20% ethanol as a staining solution.

UA-Zero Trouble Free EM Staining - 25ml bottles

Application Notes

In collaboration with Bristol University we have produced several Application Notes detailing the protocols and processes used to prepare stained specimens using both UA-Zero and Uranyl Acetate. Each Application Note contains TEM images comparing the results from UA-Zero with Uranyl Acetate.

Fixation, staining & processing of HeLa cell pellet from culture dish
Fixation, staining & processing of HeLa cells grown on coverslips
Fixation, staining & processing of kidney tissue
Tobacco Mosaic Virus (TMV) Negative staining

MSDS

UA-Zero is labelled with the appropriate hazard symbols and risk & safety phrases in accordance with current UK regulations and EC directives.

A Material Safety Data Sheets (MSDS)/Safety Data Sheets (SDS) is available to assist in the safe handling and storage of the product and the completion of any requirements under COSHH.

AGR1000 UA-Zero EM Stain MSDS

 FIND OUT MORE!

UA-Zero Discount

If you'd like to receive a 10% discount on your first bottle of UA-Zero for your own trials, please complete the form below or alternatively email: sales@agarscientific.com